The Rookie: Feds features Laura Stensen, a prominent lesbian character, depicted with dignity, complexity, and agency as a highly competent FBI agent. Her identity is normalized, and her relationships are treated with respect, contributing to an overall affirming portrayal of LGBTQ+ individuals within the series.

The show features a recurring transsexual character, Elena, a forensic scientist. She is depicted as competent, intelligent, and a valued team member, with her trans identity handled respectfully and not as a source of negative stereotypes or conflict. This portrayal contributes to a positive and affirming representation.

The show often features characters who identify as Christian, portraying their faith as a source of personal strength, moral guidance, or community connection, without broadly critiquing the religion itself.

The series includes diverse characters, some of whom are Muslim, depicting them as competent and ethical individuals within the FBI or the broader community, thereby affirming the dignity of their faith.