The animated film '100% Wolf' centers on a young werewolf's adventure and identity struggles within a traditional family and pack structure. There are no discernible LGBTQ+ characters or themes present in the story, leading to a net impact rating of N/A.

The animated film "100% Wolf" does not feature any identifiable transsexual characters or themes. Its narrative centers on a young werewolf's identity struggle and adventures, entirely unrelated to transgender experiences. Therefore, the film has no impact on the portrayal of transsexual individuals.

The film features several female characters, including Batty and Lady Highclaw. However, none of these characters are depicted engaging in or winning close-quarters physical combat against one or more male opponents. Their roles do not involve direct physical confrontation.

The film "100% Wolf" is an adaptation of the novel of the same name. A review of the main and legacy characters indicates that their genders remain consistent with their portrayals in the source material, with no instances of a character's established gender being changed.

The animated film "100% Wolf" is an adaptation of a book. There is no evidence that any character, established as one race in the source material, was portrayed as a different race in the film.