The film '2 Guns' does not include any discernible LGBTQ+ characters, themes, or storylines. The narrative focuses entirely on the actions and relationships of its heterosexual protagonists within a crime thriller context, resulting in no LGBTQ+ portrayal.

The film "2 Guns" does not feature any identifiable transsexual characters or themes. The narrative focuses entirely on its action-comedy plot involving undercover agents, drug cartels, and the CIA, without any elements related to transgender identity.

The film features Deb Rees, a DEA agent, who participates in action sequences primarily involving firearms. There are no scenes depicting her defeating one or more male opponents in direct physical combat using hand-to-hand, martial arts, or melee weapons.

The film "2 Guns" is an adaptation of a comic book series. All major characters, including Robert Trench, Michael Stigman, and Deb, maintain their established genders from the source material in the film adaptation. No character's gender was altered.