Captain Underpants: The First Epic Movie does not include any discernible LGBTQ+ characters or themes. The narrative focuses on the comedic adventures of two elementary school boys and their principal, with no elements related to queer identity present in the story.

Captain Underpants: The First Epic Movie is an animated comedy centered on two elementary school pranksters and their hypnotized principal. The film does not include any transsexual characters or explore related themes, focusing instead on childhood imagination and superhero adventures.

The film does not feature any female characters engaging in direct physical combat against male opponents. Female characters present are in non-combat roles.

The film adapts the established characters from the Captain Underpants book series. All major characters, including George, Harold, Captain Underpants/Mr. Krupp, and Professor Poopypants, retain their original canonical genders from the source material.

The film adapts the established races of its main characters, George Beard (Black) and Harold Hutchins (white), directly from the source book series. No canonical characters were portrayed as a different race.