This film does not include any identifiable LGBTQ+ characters or themes. The narrative focuses solely on the crossover mystery and superhero team-up, without engaging with queer identity or experiences in any capacity.

The animated film 'Scooby-Doo! & Batman: The Brave and the Bold' does not include any identifiable transsexual characters or themes. The narrative focuses on a crossover mystery between the Scooby gang and various DC Comics heroes and villains, without any elements pertaining to transsexual identity.

The film features established characters from both the Scooby-Doo franchise and DC Comics. All major and legacy characters, including Batman, Robin, Scooby-Doo, Shaggy, Daphne, Velma, and various villains, retain their historically canonical genders as portrayed in source material and previous adaptations.

The film features established characters from both Scooby-Doo and DC Comics. All major and legacy characters, such as the Scooby gang, Batman, and other DC heroes and villains, are depicted consistently with their long-standing canonical racial portrayals in source material and prior adaptations. No character's race was altered from its established depiction.