The film includes Harold and Howard McBride as a loving, supportive gay couple, reflecting the show's commitment to diversity. While their roles are not central to the spy plot, their positive and normalizing depiction of an LGBTQ+ family contributes to an affirming overall portrayal.

No Time to Spy: A Loud House Movie features Dana Dufresne, a transgender character. Her portrayal is integrated positively, treating her as a normal part of the community. The film reflects inclusivity and acceptance, contributing to normalized representation within the Loud House universe.

The film features Gran-Gran Myrtle, a skilled former secret agent, who repeatedly engages in and wins direct physical combat against male opponents. Her victories are achieved through hand-to-hand fighting and spy-related melee techniques.