A Bug's Life does not feature any identifiable LGBTQ+ characters or themes. The narrative and character relationships are entirely heteronormative, resulting in no portrayal of queer identity within the film's content.

A Bug's Life is an animated film centered on an ant colony and grasshoppers. The narrative and characters do not include any identifiable transsexual individuals or explore themes related to gender identity or transition, resulting in no depiction.

The film features several female characters, including Princess Atta, Dot, Rosie, and Gypsy. While some are involved in the overall conflict against the grasshoppers, none are depicted engaging in or winning close-quarters physical combat against male opponents. Their contributions are primarily strategic or involve other forms of assistance.

A Bug's Life is an original animated film with no pre-existing source material, prior installments, or historical figures. All characters were created for this film, meaning there is no prior canon from which a character's gender could have been established and subsequently changed.

A Bug's Life is an original animated film featuring anthropomorphic insect characters. There is no prior source material or established human racial identity for any character that could be subject to a race swap.