The film does not feature any identifiable LGBTQ+ characters or explicit themes. The narrative focuses on the bears' origin story and their struggle for acceptance, without incorporating specific queer identities or storylines.

We Bare Bears: The Movie does not feature any identifiable transsexual characters or themes. The narrative centers on the three bear brothers and their journey, with no elements pertaining to transgender identity or experiences present in the story.

The film features female characters like Chloe Park and Ranger Tabes. While Ranger Tabes is physically capable and participates in action sequences, her interactions with male opponents are primarily comedic or involve general physical intervention and disruption rather than clear, skilled victories in close-quarters physical combat.

The movie continues the story of the 'We Bare Bears' TV series, featuring the same established characters. All main and recurring characters retain their original, canonical genders from the prior installments, with no instances of gender alteration.

The film features the same anthropomorphic bear characters and human supporting characters as the original series. The human character, Chloe Park, maintains her established Korean-American identity, and the bears do not possess a human race to be swapped.