Blue's Big City Adventure, a children's animated musical, does not feature any identifiable transsexual characters or themes. The film's plot centers on a quest to audition for a Broadway show, and there are no elements within the narrative that depict or address transgender identity.

This film is a children's musical adventure and does not feature any scenes of physical combat. Female characters are present, but none engage in or win close-quarters physical fights against male opponents.

The film features established characters from the "Blue's Clues" franchise, such as Blue, Josh, Steve, and Joe. All returning characters maintain their original canonical genders from previous installments.

The film features the established hosts Steve, Joe, and Josh, all portrayed by their original actors. Josh, introduced in 'Blue's Clues & You!', maintains his established race. No character previously established as one race is portrayed as a different race in this movie.