The film features Bertie, a transgender woman, as a central and positively depicted character. Her identity is revealed and accepted without prejudice, portraying her with dignity, complexity, and agency. The narrative's stance is affirming, contributing positively to LGBTQ+ representation.

Arlo the Alligator Boy features Alia, a trans woman, as a positively portrayed character within Arlo's found family. Her identity is presented with dignity and respect, integrated naturally into the narrative without relying on stereotypes or negative framing. The film's overall depiction of Alia is affirming and supportive.