Spies in Disguise does not include any discernible LGBTQ+ characters or themes. The narrative focuses on a super spy and a scientist, and their evolving partnership, without touching upon queer identity in any capacity.

Spies in Disguise does not feature any identifiable transsexual characters or themes. The story centers on an animated spy adventure, and the topic of transsexual identity is not present within its narrative or character arcs.

The film features Marcy Kappel, a capable internal affairs agent, who participates in action sequences involving pursuits and tactical operations. However, there are no scenes depicting her winning direct, close-quarters physical combat against one or more male opponents through skill, strength, or martial arts.

The film "Spies in Disguise" is an original animated story, loosely based on a short film. Its characters are either new creations or maintain their original gender from the short film, with no instances of established characters being portrayed as a different gender.

Spies in Disguise is an original animated film, not an adaptation of pre-existing material with established character races. All main characters were created for this production, thus there is no prior canon from which to swap their race.