Big Hero 6 does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on themes of grief, friendship, and heroism without incorporating queer identity into its plot or character development.

Big Hero 6 does not feature any identifiable transsexual characters or themes. The story focuses on a young robotics prodigy, Hiro, and his inflatable robot companion, Baymax, as they form a superhero team to combat a masked villain, without engaging with transgender identities or experiences.

The film features female characters like Go Go Tomago and Honey Lemon who participate in action sequences. However, their combat involves technological gadgets against non-gendered microbots or as part of a team effort against the main male antagonist, Yokai. There are no scenes where a female character individually defeats one or more male opponents in direct physical combat.

The film adapts the Marvel Comics team Big Hero 6. All core characters, including Hiro, Go Go, Wasabi, Honey Lemon, and Fred, retain their established genders from the source material. No character canonically established as one gender is portrayed as another.