Merry Little Batman does not feature explicit LGBTQ+ characters or themes. The narrative focuses on family, heroism, and holiday spirit, particularly the father-son relationship between Batman and Damian Wayne. Any potential queer coding in character mannerisms is noted as an homage to classic animation, not an explicit LGBTQ+ portrayal.

Available information indicates that 'Merry Little Batman' does not explicitly feature or highlight transsexual characters or themes within its narrative. Search results discuss broader LGBTQ+ representation in Batman media but provide no specific details regarding transsexual portrayals in this particular film, leading to a determination of no identifiable depiction.

The film features Poison Ivy engaging in hand-to-hand and melee combat with male opponents like Damian Wayne and Bane. However, the provided information does not detail any specific instances where Poison Ivy or any other female character is shown to be victorious over male opponents in direct physical combat.

The film's major characters, including Damian Wayne, Bruce Wayne, Alfred Pennyworth, and various villains, maintain their traditional genders as established in the DC Comics universe. No characters were portrayed with a different gender than their source material.