This animated Scooby-Doo film does not feature any identifiable LGBTQ+ characters or themes. The story centers on the gang's typical mystery-solving antics, focusing on a supernatural adventure in Japan without incorporating any queer representation.

This Scooby-Doo animated film does not feature any identifiable transsexual characters or themes. The story centers on the gang's adventure in Japan, solving a mystery involving a samurai ghost and a martial arts tournament, without engaging with transgender identity in any capacity.

The film features the established Scooby-Doo characters, all retaining their canonical genders. No new or adapted characters are identified as having their gender changed from prior canon or historical record.

The main characters (Shaggy, Fred, Daphne, Velma) are consistently depicted as white, aligning with their established canon. New characters introduced in the film do not constitute race swaps.