Spirit: Stallion of the Cimarron does not feature any identifiable LGBTQ+ characters or themes within its narrative. The story centers on a wild horse's quest for freedom and his experiences with humanity, resulting in no portrayal of LGBTQ+ elements.

Spirit: Stallion of the Cimarron does not feature any identifiable transsexual characters or themes. The narrative centers on a wild horse's quest for freedom, with no elements related to gender identity or transgender experiences.

The film primarily features male characters in combat or action roles. While female animals are present, no female character, human or animal, engages in and wins direct physical combat against one or more male opponents using skill, strength, or martial arts.

Spirit: Stallion of the Cimarron is an original animated film with no pre-existing source material or legacy characters. All characters were created specifically for this movie, thus precluding any gender swaps from prior canon.

The film features original characters, primarily animals, and human characters whose races were established within the film's initial creation. There is no prior source material or established canon from which character races were altered.