The film "3 Ninjas: High Noon at Mega Mountain" does not feature any identifiable LGBTQ+ characters or themes. Its narrative focuses on children's action-comedy within a theme park setting, with no elements related to queer identity or experiences.

The film "3 Ninjas: High Noon at Mega Mountain" does not feature any identifiable transsexual characters or themes. Its plot centers on a children's action-comedy premise, completely devoid of content related to transsexual identity or experiences. Therefore, the net impact on the portrayal of transsexual characters and themes is N/A.

The film focuses on three young male protagonists and their grandfather engaging in physical combat against male antagonists. No significant female characters are depicted winning close-quarters physical fights against male opponents.

This film is a sequel in an established franchise. All major characters, including the three ninja brothers and Grandpa, retain their canonically established genders from previous installments. No characters originally established as one gender are portrayed as a different gender.

The film features the established characters Rocky, Colt, and Tum-Tum, who are consistently portrayed by white actors across the series, including this installment. No character's race was changed from previous canonical depictions.