The film '65' does not feature any identifiable LGBTQ+ characters or themes. Its narrative is solely centered on a sci-fi survival story, thus rendering the net impact on LGBTQ+ portrayal as N/A.

The film '65' (2023) does not feature any transsexual characters or engage with themes related to gender identity. Its plot is centered on a sci-fi survival story, resulting in no portrayal or representation of the transsexual community within its narrative.

The film features Koa, a young female character, who is primarily a survivor and not a combatant. She does not engage in or win close-quarters physical combat against any male opponents. The primary threats are dinosaurs, and the male protagonist handles most combat.

The film "65" is an original story with characters created specifically for this production. There is no prior source material, historical record, or established canon from which characters' genders could have been altered.

The film "65" is an original science fiction story with characters created specifically for this movie. There is no prior source material, historical record, or established canon to define the characters' races before their portrayal in this film, thus precluding a race swap.