Aliens vs. Predator: Requiem does not feature any identifiable LGBTQ+ characters or themes. The story is solely centered on the sci-fi horror conflict, rendering the portrayal of LGBTQ+ elements as N/A due to their complete absence from the narrative.

The film "Aliens vs Predator: Requiem" does not feature any identifiable transsexual characters or themes. Its narrative focuses entirely on the conflict between extraterrestrial species and human survival, without exploring aspects of gender identity.

The film features female characters, primarily Kelly O'Brien, who engage in combat. However, their victories are achieved through the use of firearms against alien creatures, not through close-quarters physical combat against male opponents. No female character is depicted winning a hand-to-hand or melee fight against a male.

All significant characters in "Aliens vs. Predator: Requiem" are either new creations for this film or consistent with their established species characteristics. No character previously established as one gender in prior canon is portrayed as a different gender.

The film introduces new human characters and creatures. There are no instances of a character who was canonically, historically, or widely established as one race being portrayed as a different race.