The film "Batman Beyond: Return of the Joker" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the conflict between Batman (Terry McGinnis) and the resurrected Joker, exploring themes of legacy and trauma within the established DC Animated Universe without incorporating queer representation.

Batman Beyond: Return of the Joker does not feature any identifiable transsexual characters or themes. The narrative focuses on the conflict between Batman (Terry McGinnis) and the resurrected Joker, exploring themes of legacy, trauma, and justice within the futuristic Gotham City setting, without engaging with transgender identity.

The film features female characters like Melanie Walker (Ten) and the Dee Dee Twins who engage in physical combat. While skilled, these characters do not achieve clear victories over one or more male opponents in direct physical combat. Their primary combat interactions are either against the male protagonist, where they are eventually subdued or outmaneuvered, or against other female characters.

The film features established characters like Batman (Terry McGinnis and Bruce Wayne), The Joker, and Commissioner Barbara Gordon, all of whom retain their canonically established genders. No legacy characters from the source material are portrayed with a different gender.

This animated film features established DC characters like Batman, Bruce Wayne, and The Joker. All major characters are portrayed consistent with their long-standing canonical race from comics and previous animated series, with no instances of a character's race being changed.