Ben 10: Secret of the Omnitrix is an animated sci-fi adventure film. It does not contain any identifiable LGBTQ+ characters, relationships, or themes. Consequently, there is no portrayal of LGBTQ+ elements to evaluate within the narrative.

The film "Ben 10: Secret of the Omnitrix" does not feature any identifiable transsexual characters or themes. The narrative focuses on sci-fi action and adventure, with no elements pertaining to transgender identity or experiences.

While female characters like Gwen Tennyson are present and participate in combat, their victories against male opponents primarily rely on the use of their unique energy-based superpowers. There are no clear instances where a female character defeats one or more male opponents through skill, strength, or martial arts in close-quarters physical combat.

The film features established characters from the Ben 10 animated series, all of whom retain their original canonical genders. No characters previously established as one gender are portrayed as a different gender.

This animated film is a direct continuation of the original Ben 10 series. All established human characters, such as Ben, Gwen, and Grandpa Max, maintain their original racial depictions from the source material. No characters who were canonically established as one race are portrayed as a different race.