The animated film "Hulk vs. Wolverine" does not feature any identifiable LGBTQ+ characters or themes. The plot is exclusively focused on action and the conflict between its titular characters and other mutants, resulting in no portrayal of queer identity within its storyline.

Hulk vs. Wolverine is an animated action film centered on the titular characters' conflict and Wolverine's past. The movie does not feature any identifiable transsexual characters or explore related themes within its storyline.

The film features female characters like Lady Deathstrike and Mystique who engage in combat. However, Lady Deathstrike does not achieve clear physical victories over male opponents, and Mystique's combat style relies more on her shapeshifting abilities rather than direct physical confrontation. No female character is shown to defeat one or more male opponents in close-quarters physical combat.

All major characters in "Hulk vs. Wolverine," including Hulk, Wolverine, Lady Deathstrike, and Professor X, maintain their established canonical genders from the Marvel Comics source material.

The film features established Marvel characters Hulk (Bruce Banner) and Wolverine (Logan), both of whom are depicted as white, consistent with their long-standing comic book portrayals. No characters were found to have their race changed from their canonical or historically established depictions.