Batman vs Teenage Mutant Ninja Turtles does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on action and the crossover event between the two iconic franchises, without incorporating elements related to queer identity.

The film "Batman vs Teenage Mutant Ninja Turtles" does not feature any identifiable transsexual characters or themes. Its narrative is solely focused on the action-adventure crossover between the titular heroes and their respective villains. There are no plot points or character arcs that depict or explore transsexual identity, resulting in a rating of N/A for no depiction.

The film features established characters from both the Batman and Teenage Mutant Ninja Turtles universes. All major characters, such as Batman, the Turtles, April O'Neil, and the villains, retain their canonically established genders from previous iterations.

All major human characters, such as Batman, Joker, Harley Quinn, and Shredder, are voiced by actors whose race aligns with the characters' established canonical portrayals. The Teenage Mutant Ninja Turtles are anthropomorphic and thus do not have a human race.