The film does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the main trio's adventure, with all relationships and identities presented within a heteronormative framework, resulting in no depiction of LGBTQ+ elements.

The film 'Ed, Edd n Eddy's Big Picture Show' does not include any identifiable transsexual characters or themes. The narrative focuses on the misadventures of the main characters without engaging with transsexual identity or related issues.

The movie does not contain any action or adventure elements.

This animated crossover special features established characters from various Cartoon Network shows, including Ed, Edd n Eddy. There is no evidence or widely known information suggesting that any canonically male or female character was portrayed as a different gender in this installment.

This animated film is a direct continuation of the "Ed, Edd n Eddy" series. All established characters maintain their original animated designs and visual depictions, with no changes to their race from the source material.