The film "Night of the Living Doo" does not feature any identifiable LGBTQ+ characters or themes. Its narrative focuses on a horror-comedy parody of the Scooby-Doo franchise, with no elements related to queer identity or experiences, resulting in no depiction to evaluate.

The film 'Night of the Living Doo' does not include any identifiable transsexual characters or themes. Its plot focuses entirely on a comedic horror scenario involving a zombie outbreak within the Scooby-Doo universe.

The animated short features female characters Daphne Blake and Velma Dinkley. However, the film is a horror-comedy parody where the characters primarily react to threats and attempt to solve a mystery, rather than engaging in direct physical combat. There are no scenes depicting female characters defeating male opponents in close-quarters physical fights.

The film features the established Scooby-Doo characters, all of whom retain their canonical genders from previous iterations. No characters widely established as one gender are portrayed as a different gender in this production.

The animated special features the established Scooby-Doo characters, all of whom are depicted with their traditional racial appearances, consistent with their long-standing canon. No character's race was altered from previous portrayals.