This Scooby-Doo animated special does not include any explicit LGBTQ+ characters or themes. The narrative focuses on the traditional mystery-solving adventures of the gang, with no elements suggesting queer identity or experiences. Therefore, the film has no discernible impact on LGBTQ+ representation.

The film "Scooby-Doo! Haunted Holidays" does not feature any identifiable transsexual characters or themes. The narrative focuses on a typical Scooby-Doo mystery during the holiday season, without engaging with gender identity topics. Therefore, there is no portrayal to evaluate.

The film features female characters Daphne Blake and Velma Dinkley who participate in the mystery. However, their roles do not include scenes where they physically defeat male opponents in close-quarters combat. The villain is apprehended through traps and teamwork, not individual physical victories by female characters.

This Scooby-Doo animated special features the classic Mystery Inc. gang—Fred, Daphne, Velma, Shaggy, and Scooby-Doo—all portrayed with their established canonical genders. No characters from prior installments or source material have their gender changed.

The film features the established Scooby-Doo characters (Shaggy, Fred, Daphne, Velma), all of whom are depicted as white, consistent with their long-standing canonical portrayals across the franchise. No character's race deviates from their established depiction.