The film "Scooby-Doo! Pirates Ahoy!" does not include any explicit or implicitly identifiable LGBTQ+ characters or themes. The narrative focuses solely on the mystery-solving adventures of the Scooby-Doo gang, with no elements pertaining to queer identity or experiences.

The film "Scooby-Doo! Pirates Ahoy!" does not include any identifiable transsexual characters or explore related themes within its plot. The narrative focuses on a typical Scooby-Doo mystery without any depiction of transgender identity or issues.

The film primarily features the Scooby-Doo gang solving a mystery involving ghost pirates. While female characters like Daphne and Velma are present and participate in the adventure, their roles do not include direct physical combat where they defeat male opponents through skill, strength, or martial arts. Victories are achieved through solving the mystery and unmasking the villains.

This animated film features the classic Scooby-Doo gang, who retain their long-established canonical genders. No characters from source material or previous installments are portrayed as a different gender.

The film features the established core Scooby-Doo characters (Fred, Daphne, Velma, Shaggy) who are consistently portrayed as white, aligning with their long-standing animated depictions. No canonical characters have their race altered in this installment.