This Scooby-Doo animated film does not include any explicit LGBTQ+ characters or themes. The narrative focuses on the Mystery Inc. gang solving a mystery in the Himalayas, with no elements related to queer identity or experiences present in the story.

The film "Chill Out, Scooby-Doo!" does not feature any identifiable transsexual characters or themes. Its plot is a standard Scooby-Doo mystery, and there are no elements within the story that depict or allude to transgender identity or experiences.

The film primarily features comedic chases, traps, and mystery-solving. Female characters like Daphne and Velma participate in the adventure, but there are no scenes depicting them engaging in or winning direct physical combat against one or more male opponents.

The film features the established Scooby-Doo characters (Fred, Daphne, Velma, Shaggy, Scooby) who all retain their long-standing canonical genders. No pre-existing characters from the Scooby-Doo franchise are portrayed with a different gender in this installment.

The film features the core Scooby-Doo characters (Fred, Daphne, Velma, Shaggy) who maintain their long-established racial depictions from prior canon. No established character's race was altered for this installment.