The film "Scooby-Doo on Zombie Island" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses entirely on the mystery, the supernatural elements, and the main characters' adventure, with no elements pertaining to queer identity or experiences.

The film "Scooby-Doo on Zombie Island" does not feature any identifiable transsexual characters or themes. The narrative focuses on the Scooby gang investigating a haunted island and encountering supernatural creatures, with no elements related to transgender identity or experiences.

The movie does not contain any action or adventure elements.

The core Mystery Inc. characters (Scooby-Doo, Shaggy, Fred, Daphne, Velma) retain their established genders from previous iterations of the franchise. No other canonically established characters from the Scooby-Doo universe are portrayed with a different gender in this film.

The film features the established Mystery Inc. characters, who are consistently portrayed as white, aligning with their long-standing canonical depictions. All other significant characters are original to this specific film and do not have prior racial portrayals to be altered.