Scooby-Doo! and the Cyber Chase, an animated film from 2001, does not include any identifiable LGBTQ+ characters or themes. The story focuses on the Mystery Inc. gang's adventure inside a video game, with no elements related to queer identity or experiences present in the plot or character arcs.

Scooby-Doo! and the Cyber Chase, an animated children's film, does not include any identifiable transsexual characters or themes. The narrative focuses on the Scooby gang solving a mystery within a video game, without engaging with LGBTQ+ identity.

The film features Daphne Blake and Velma Dinkley as key characters, but their roles do not involve direct physical combat victories against male opponents. The gang primarily solves mysteries and evades digital threats within a video game environment.

The film features the established Scooby-Doo characters, who retain their canonical genders. No previously established characters from the franchise are portrayed with a different gender in this installment.

The main characters (Fred, Daphne, Velma, Shaggy, Scooby-Doo) are consistently portrayed as they have been in prior animated iterations. There are no instances where a canonically established character's race has been altered.