The film "Bolt" does not feature any identifiable LGBTQ+ characters or themes within its narrative. As such, there is no portrayal to evaluate regarding queer identity, relationships, or experiences, resulting in a 'Not Applicable' rating for its net impact on LGBTQ+ representation.

Bolt is an animated adventure film centered on a dog who believes he has superpowers. The story does not feature any identifiable transsexual characters or themes, nor does it touch upon gender identity in any capacity. The narrative is entirely focused on the animal protagonists and their journey.

The film features Penny, a young girl, and Mittens, a cat. Neither character engages in direct physical combat against male opponents where they are shown to be victorious through skill or strength. Their roles do not involve such combat scenarios.

Bolt is an original animated film featuring characters created specifically for this movie. There is no prior source material, historical figures, or previous installments from which character genders could have been altered.

Bolt is an original animated film with no pre-existing source material or historical figures. All characters were created for this specific movie, thus there is no prior established race for any character to be swapped from.