The Great Mouse Detective does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on a classic detective story within a Victorian London setting, without incorporating elements related to queer identity or experiences.

The Great Mouse Detective does not feature any identifiable transsexual characters or themes. The narrative focuses on a classic mystery adventure within a mouse society, with no elements related to transgender identity or experiences.

The film does not feature any female characters engaging in or winning direct physical combat against one or more male opponents. Female characters are present but do not participate in action sequences involving close-quarters fighting.

The film adapts characters from the "Basil of Baker Street" book series, which are pastiches of Sherlock Holmes. All major characters derived from established sources, such as Basil (Sherlock Holmes) and Dr. Dawson (Dr. Watson), retain their original male gender. No established character undergoes a gender change.

The film's characters are anthropomorphic mice, not humans. The concept of human racial categories and subsequent race swaps does not apply to these characters, as their species is mouse.