James and the Giant Peach does not feature any discernible LGBTQ+ characters or themes. The story centers on a young boy's escape from his cruel aunts and his magical adventure with anthropomorphic insects, with no elements related to queer identity present in the narrative.

James and the Giant Peach does not feature any explicit or implicit transsexual characters or themes. The story centers on a young boy's escape from his cruel aunts and his fantastical journey with anthropomorphic insects, with no elements related to transgender identity or experiences.

The film features female characters such as Miss Spider, Mrs. Ladybug, Aunt Spiker, and Aunt Sponge. However, none of these characters engage in or win direct physical combat against one or more male opponents through skill, strength, or martial arts.

The 1996 film adaptation of Roald Dahl's novel maintains the original genders for all its main and supporting characters, including James, his aunts, and the insect companions. No character established as one gender in the source material is portrayed as a different gender in the movie.

The film adapts Roald Dahl's novel, maintaining the established racial depictions of its human characters. No character canonically, historically, or widely established as one race is portrayed as a different race.