The film "Rise of the Guardians" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on mythical figures protecting children's beliefs, with no elements related to queer identity or experiences present in the story.

Rise of the Guardians does not feature any identifiable transsexual characters or themes. The narrative centers on established mythical figures and their adventures, with no elements related to gender identity or transition present in the story or character portrayals.

The film features Toothiana, a powerful female character, who primarily uses flight and speed. While she participates in action sequences against the male villain Pitch Black and his nightmare creatures, there are no scenes where she physically defeats one or more male opponents in close-quarters combat using skill, strength, or martial arts.

The film adapts well-known mythological figures such as Santa Claus, the Easter Bunny, and Jack Frost. All major characters retain their traditionally established genders from folklore and previous depictions, with no instances of a character's gender being altered from their source material.

The film adapts various folklore figures such as Jack Frost, Santa Claus, and the Easter Bunny. For these characters, their on-screen portrayals align with traditional depictions or their race was not canonically established in source material. No character established as one race in prior canon is portrayed as a different race in the film.