The film "Americana, 2025" does not feature any identifiable LGBTQ+ characters, relationships, subplots, or themes. Available information indicates the narrative centers on a crime thriller involving a Lakota Ghost Shirt and various outsiders, with no noted queer elements in its plot or character descriptions.

Based on all available information, 'Americana' (2025) does not appear to feature any identifiable transsexual characters or themes. The film's narrative focuses on other character arcs and plot points, such as a stuttering waitress, a woman fleeing an abusive relationship, and a veteran cowboy, all clashing over a Lakota Ghost Shirt artifact. Therefore, the film receives an N/A rating for its portrayal of transsexual characters and themes.

Available information for Americana (2025) does not document any scenes where female characters, such as Penny Jo Poplin or Mandy Starr, engage in and are victorious in direct physical combat against one or more male opponents. The plot involves violence, but no specific instances of female characters winning close-quarters physical fights against men are noted.

The film "Americana" features an original ensemble of characters in a new story. There is no information indicating that any character is adapted from prior source material or historical record with a different gender.

All major characters in "Americana, 2025" are original creations for this film, with no prior source material identified. Therefore, no character was canonically, historically, or widely established as one race and then portrayed as a different race.