The film features Renee Montoya, a prominent lesbian character, depicted with dignity and agency as a determined detective and eventual hero. Her sexuality is casually integrated into her character without being a source of conflict or negative stereotypes, contributing to a respectful and affirming portrayal of an LGBTQ+ individual.

The film features multiple female characters, including Harley Quinn, Black Canary, and Huntress, who repeatedly engage in and win close-quarters physical fights against numerous male opponents, demonstrating skill and strength.

Black Canary, a character canonically established as white in DC Comics, is portrayed by a Black actress in the film, constituting a race swap.