The film "Blade" does not feature any identifiable LGBTQ+ characters or themes. The narrative is solely focused on action and horror within a vampire-hunting context, without exploring queer identities or experiences in any capacity.

The film "Blade" does not feature any identifiable transsexual characters or themes. The narrative focuses entirely on its primary plot elements involving vampires and vampire hunters, without engaging with transgender identity in any capacity.

The film does not feature any female characters who engage in or win close-quarters physical combat against one or more male opponents. The primary female character, Dr. Karen Jenson, is a scientist and not a combatant.

The film's main characters, Blade and Deacon Frost, retain their established male gender from the Marvel Comics source material. Other significant characters were either new creations for the movie or did not have a widely established gender in prior canon that was subsequently changed.

The character Blade was canonically established as a Black man in Marvel Comics. In the 1998 film, he is portrayed by a Black actor, Wesley Snipes, aligning with the source material. No other significant characters in the film represent a race swap from their established comic book origins or historical depictions.