Brotherhood of the Wolf does not include any explicit or implicitly identifiable LGBTQ+ characters or themes. The narrative focuses on historical mystery, action, and heterosexual romantic subplots, with no elements related to queer identity or experiences.

Brotherhood of the Wolf does not feature any identifiable transsexual characters or themes. The film's plot and character arcs are entirely unrelated to gender identity, resulting in no portrayal, either positive or negative, of transsexual experiences.

The film features several female characters, but none are depicted engaging in or winning close-quarters physical combat against male opponents. Their roles do not involve direct martial or hand-to-hand confrontations.

The film is a highly fictionalized account of a historical legend, introducing many original characters. No established historical figures or characters from prior canon are portrayed with a different gender than their original depiction or historical record.

The film introduces an original character, Mani, an Iroquois warrior, who was not established as a different race in prior canon or history. Other main characters are portrayed consistently with their historical or canonical race. Therefore, no race swaps occurred.