Titane features a protagonist whose bisexuality and gender fluidity are integral to her complex identity and transformative journey. The film explores themes of unconventional love and family through a paternal figure's unconditional acceptance of a gender-non-conforming individual, ultimately affirming non-normative identities and relationships within its unique, visceral narrative.

The film features a cisgender woman adopting a male identity and presentation as a survival mechanism, which becomes central to the plot. While a grieving father offers unconditional acceptance to this adopted identity, the narrative primarily uses gender fluidity as a plot device for themes of body horror and unconventional family, rather than exploring or affirming transsexual identity itself.