Captain America: Civil War does not feature any identifiable LGBTQ+ characters or themes. The film's narrative is centered on the conflict between Captain America and Iron Man over government oversight of superheroes, and the re-emergence of the Winter Soldier's past.

Captain America: Civil War does not feature any identifiable transsexual characters or themes. The film's plot and character arcs are entirely centered on the superhero conflict and its political ramifications, leading to a determination of N/A for transsexual portrayal.

The film faithfully adapts its Marvel Comics source material, with all established male and female characters retaining their original genders. No canonical character underwent a gender change.

The film's main and legacy characters, including Captain America, Iron Man, and Black Widow, are portrayed by actors whose race aligns with their established comic book or prior cinematic depictions. No instances of a character canonically established as one race being portrayed as a different race were identified.