Charlotte's Web 2: Wilbur's Great Adventure, an animated children's film, does not include any discernible LGBTQ+ characters or themes. Therefore, it offers no specific portrayal, positive or negative, of queer identities or experiences.

The film "Charlotte's Web 2: Wilbur's Great Adventure" is an animated children's movie centered on anthropomorphic farm animals. It does not contain any identifiable transsexual characters or themes, nor does it explore topics related to gender identity within its narrative.

The film is an animated children's movie where conflicts are resolved primarily through teamwork, cleverness, and non-violent means. No female characters are depicted engaging in or winning close-quarters physical combat against male opponents using skill, strength, or martial arts.

This film is a direct sequel to the 1973 animated adaptation of E.B. White's novel. All returning characters maintain their established genders, and new characters introduced in the sequel do not constitute gender swaps.

The film's main characters are animals, which do not possess human racial characteristics. The human characters, such as Fern and the Zuckermans, maintain their established racial depiction from the original novel and prior animated film.