The film does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on the Nohara family's move to Mexico and their adventure battling attacking cacti, without incorporating elements related to queer identity or experiences.

This film, a Crayon Shin-chan adventure, focuses on the Nohara family's move to Mexico and their encounter with sentient cacti. There are no identifiable transsexual characters or themes present in the narrative, nor are there any elements that could be interpreted as such. Therefore, the film has no depiction of transsexual identity.

The film's primary antagonists are sentient cacti, which are not gendered. No female character is depicted engaging in or winning direct physical combat against one or more male opponents, whether human or otherwise gendered, through skill, strength, or martial arts.

The film features the established characters of the Crayon Shin-chan franchise, such as Shinnosuke, Misae, and Hiroshi Nohara, all retaining their canonical genders. No legacy characters are portrayed as a different gender.

This animated film is part of the Crayon Shin-chan franchise, which consistently features Japanese characters. There is no evidence or widely known information suggesting that any established character's race was altered from their canonical depiction in this installment.