Lupin III: The First does not feature any identifiable LGBTQ+ characters or themes. The film's narrative centers on the main characters' adventure to uncover the secrets of the Bresson Diary, with no elements related to queer identity or experiences present in the story.

The available information for Lupin III: The First does not indicate the presence of any identifiable transsexual characters or themes. Consequently, there is no depiction to assess within the given framework, leading to an N/A rating.

The film features female characters such as Fujiko Mine and Laetitia. While they are involved in the plot and demonstrate resourcefulness, neither character engages in or wins close-quarters physical combat against one or more male opponents. Their contributions are primarily through stealth, intelligence, or other means.

The film features the established characters of the Lupin III franchise, such as Lupin III, Jigen, Goemon, Fujiko, and Zenigata, all of whom retain their canonically established genders from the original manga and previous adaptations. No legacy character's gender was altered.

Lupin III: The First is a Japanese animated film based on a long-running Japanese manga and anime series. The characters maintain their established appearances and implied ethnicities consistent with prior canon, with no instances of a character originally depicted as one race being portrayed as a different race.