No LGBTQ+ characters or themes appear in the film.

No transgender characters or themes appear in the film. The story centers on Lupin III and his team's adventure against an immortal antagonist on a mysterious island, with no elements related to transsexual identities.

Fujiko Mine, the main female character, does not engage in physical combat against male opponents. She is captured for sacrifice, rescued by Goemon, and escapes without fighting. Salifa clones appear but take no combat roles. The film features action for male characters but none matching the criteria for females.

The film features the standard Lupin III ensemble with all major characters portrayed in their canonical genders: Lupin III, Jigen, Goemon, and Fujiko as male, male, male, and female, respectively. New characters like Salifa, a female clone of the male Mamo, do not constitute a gender swap of an established character.

This animated film portrays the core Lupin III characters with their established Japanese depictions, using the original Japanese voice cast. New characters are original creations without prior racial canon. No instances of race swaps appear.