The film does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on a bombing plot and the past of the police academy classmates, with no explicit or implicit queer representation present in the story.

The film 'Detective Conan: The Bride of Halloween' does not feature any identifiable transsexual characters or themes. The plot centers on a crime investigation and character backstories, with no elements related to transgender identity or experiences.

The film features physically capable female characters like Ran Mouri and Miwako Sato. While Ran performs impressive feats of strength and martial arts, these are primarily used for defense, escape, or breaking environmental obstacles, not for defeating male opponents in direct physical combat. Miwako Sato's actions are primarily tactical and involve firearms.

The film features established characters from the Detective Conan series, all of whom retain their canonical genders as portrayed in previous installments and source material. No character established as one gender is depicted as a different gender.

This anime film features characters consistently portrayed as East Asian (Japanese), aligning with their established depictions in the long-running manga and anime franchise. There are no instances of characters canonically or previously established as one race being depicted as a different race.