The film 'Detective Conan: The Private Eyes' Requiem' does not feature any discernible LGBTQ+ characters or themes. Its plot is centered on a mystery and action, with no elements related to queer identity or experiences.

The film "Detective Conan: The Private Eyes' Requiem" does not feature any identifiable transsexual characters or themes. The narrative focuses on a mystery involving a bombing at a theme park and the main cast's efforts to solve it, without engaging with transgender identity in any capacity.

The film features female characters like Ran Mouri, who possesses martial arts skills, but there are no scenes depicting her or any other female character directly defeating one or more male opponents in close-quarters physical combat. Action sequences involving female characters do not meet the specified criteria for victory against male combatants.

The film features established characters from the Detective Conan franchise, all of whom retain their canonical genders as depicted in the original manga and prior adaptations. No characters established as one gender are portrayed as a different gender in this installment.

This Japanese animated film is based on a Japanese manga series. All characters maintain their established East Asian racial depiction consistent with the source material. There are no instances of characters canonically established as one race being portrayed as a different race.