Made in Abyss: Dawn of the Deep Soul does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on dark fantasy adventure, exploration, and the ethical dilemmas of scientific pursuit, without incorporating elements related to sexual orientation or gender identity.

Made in Abyss: Dawn of the Deep Soul does not feature any identifiable transsexual characters or themes. The story centers on the perilous journey into the Abyss, exploring themes of sacrifice, humanity, and the unknown, without addressing gender identity.

The film features female characters like Riko, Nanachi, and Faputa. Riko and Nanachi are not direct combatants. Faputa possesses immense power as a Narehate, engaging in destructive acts against various entities and Bondrewd. However, her combat style relies on overwhelming monstrous abilities rather than human-like physical skill or martial arts against male opponents.

The film is a direct adaptation of the manga, and all major characters retain their established genders from the source material. No character canonically established as one gender is portrayed as a different gender.

The film is an animated adaptation of a Japanese manga set in a fantasy world. Characters are depicted in a stylized anime aesthetic, where real-world racial categories are not explicitly established or canonically defined in the source material. Thus, no race swap occurs.