The film features Mr. 2 Bon Clay, a gender non-conforming character, whose portrayal is largely positive. He is depicted with dignity, agency, and complexity, demonstrating loyalty and making heroic sacrifices. His unique identity is presented as a source of strength and individuality rather than ridicule or villainy, contributing to an affirming net impact.

The film features Nami, who uses her staff-like weapon and agility to defeat male opponents in close-quarters combat. Nico Robin also uses her unique ability to physically overpower and incapacitate male characters.

The film prominently features a 'chosen family' (the Straw Hat Pirates) with fluid gender roles and a strong emphasis on loyalty and mutual support, normalizing and celebrating this non-traditional family structure as its central positive force. While a respectful biological father-daughter relationship is also depicted, the dominant framing favors alternative family arrangements.