The film features Emporio Ivankov, a prominent 'Okama' character, who is depicted as a powerful and respected leader. Ivankov actively participates in the main conflict, using their abilities to aid the protagonists. Their gender expression is presented as an integral part of their strong identity, without being a source of mockery or degradation within the narrative, thus contributing to a net positive portrayal.

The film features multiple female characters who engage in direct physical combat against male opponents. Nico Robin uses her Devil Fruit power to physically incapacitate groups of male adversaries. Boa Hancock defeats male opponents with powerful kicks. Carrot also uses her enhanced physical abilities to overcome male adversaries.

The film primarily portrays and celebrates a 'chosen family' unit among the pirate crew, emphasizing loyalty and shared adventure over traditional biological or marital family structures.