The film portrays LGBTQ+ characters through nuanced queer-coding, emphasizing emotional depth, complex identities, and respectful relationships. Characters like Ymir, Historia, Levi, Erwin, and Hange contribute to positive visibility by challenging heteronormative norms within a survival-driven world, making their identities part of their complex humanity rather than the central plot focus.

The film features Mikasa Ackerman, a highly skilled soldier, who engages in and wins close-quarters physical combat using blades and ODM gear against multiple male opponents during a critical battle.

The film utilizes Christian motifs such as sacrifice, sin, redemption, and persecution to enrich its narrative. These themes are presented with gravity and depth, aligning the narrative with the dignity of these concepts and fostering audience sympathy for the persecuted, reflecting a positive engagement with Christian themes.

Judaism is referenced through the theme of persecution, likening the plight of Ymir's descendants to historical Jewish persecution. This narrative framing encourages sympathy for the victimized group, thereby affirming the historical experience and resilience associated with the Jewish faith.