Gurren Lagann the Movie: The Lights in the Sky Are Stars does not include identifiable LGBTQ+ characters or themes. The narrative focuses on its core science fiction and action elements without exploring queer identities or relationships.

Gurren Lagann the Movie: The Lights in the Sky Are Stars does not feature identifiable transsexual characters or themes within its narrative. The film focuses on themes of human perseverance, evolution, and large-scale mecha combat, without engaging with transsexual identity or experiences. No specific plot points or character arcs related to transsexual themes are present.

This film is a compilation of an existing anime series. All major characters retain their established genders from the original series. No characters who were canonically male or female are portrayed as a different gender.

The animated film is a compilation of an existing anime series. The characters maintain their original animated designs and visual portrayals from the source material. No characters established as one race in the original series are depicted as a different race in this movie.